Overview

  • Product nameAnti-VP16 tag antibody
  • Description
    Rabbit polyclonal to VP16 tag
  • SpecificityThis antibody was raised against an immunogen that is predicted to react with HSV-1. We do not expect this product to react with HSV-2. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Immunogen

    Synthetic peptide derived from within the activation domain (amino acids 413-490) of HSV VP16.

  • Positive control
    • This antibody recognises GAL4-VP16 recombinant fusion protein (30kDa) in Western blot.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 30 kDa.Can be blocked with VP16 tag peptide (ab13406).
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Anti-VP16 tag antibody images

  • ab4808 at a 1/100 dilution staining mouse brain sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using an Alexa-Fluor 488 ® conjugated Donkey anti-Rabbit IgG.

    This image is courtesy of an Abreview submitted on 10 March 2006.

    See Abreview

  • Lane 1 : Marker
    Lanes 2 - 3 : Anti-VP16 tag antibody (ab4808) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : GAL4-VP16 human recombinant E.coli
    Lane 3 : GAL4-VP16 human recombinant E.coli with VP16 tag peptide (ab13406) at 1 µg/ml

    Secondary
    Lanes 2 - 3 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution


    Performed under reducing conditions.

References for Anti-VP16 tag antibody (ab4808)

This product has been referenced in:
  • Lemonidis K  et al. Identification of a Novel Sequence Motif Recognized by the Ankyrin Repeat Domain of zDHHC17/13 S-Acyltransferases. J Biol Chem 290:21939-50 (2015). Read more (PubMed: 26198635) »
  • Grefen C The split-ubiquitin system for the analysis of three-component interactions. Methods Mol Biol 1062:659-78 (2014). WB . Read more (PubMed: 24057391) »

See all 11 Publications for this product

Product Wall

Thank you for your inquiry. Unfortunately, I am sorry this antibody has not been tested for detection of the endogenous full length tetracycline reverse transcriptional activator protein. It is quite likely to detect, as the VP16 tag is from the C ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Rat brain)
Specification Rat brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton X-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (chinese hamster ovaries)
Loading amount 20 µg
Specification chinese hamster ovaries
Treatment cells were overexpressing a VP16 fusion protein
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 02 2008

Thank you for your enquiry. I am sorry to hear that your customer has not been able to obtain satisfactory results using this antibody. I appreciate their frustration. I am assuming that your customer is using a transgenic mouse with a camkII-tTa-V...

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Thank you for your enquiry. The VP16 activation domain is amino acids 413-490. The peptide that this antibody was raised to lies within this region. I hope this information helps, please do not hesitate to contact us if you need any more advice ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Mar 10 2006

Thank you for your enquiry. This antibody recognizes VP16 only. The immunogen was derived from a synthetic peptide derived from residues 450 - 550 of HSV VP16 tag. I hope this information helps, please do not hesitate to contact us if you need a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"