Overview

  • Product nameAnti-VPAC2 antibody
    See all VPAC2 primary antibodies
  • Description
    Rabbit polyclonal to VPAC2
  • Tested applicationsSuitable for: ICC/IF, ICC, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    LQFHRGSRAQSFLQTETSVI

    conjugated to KLH, corresponding to amino acids 419-438 of Human VIP Receptor 2

  • Positive control
    • Membrane preparations for HEK 293 cells stably expressing VPAC2.

Properties

Applications

Our Abpromise guarantee covers the use of ab28624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ICC 1/1000.
IHC-P 1/500.
WB 1/1000. Detects a band of approximately 50-60 kDa.

Target

  • FunctionThis is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.
  • Tissue specificityExpressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Helodermin-preferring VIP receptor antibody
    • PACAP type III receptor antibody
    • PACAP-R-3 antibody
    • PACAP-R3 antibody
    • Pituitary adenylate cyclase-activating polypeptide type III receptor antibody
    • Vasoactive intestinal polypeptide receptor 2 antibody
    • VIP Receptor 2 antibody
    • VIP-R-2 antibody
    • Vipr2 antibody
    • VIPR2_HUMAN antibody
    • VPAC2 receptor antibody
    see all

Anti-VPAC2 antibody images

  • ab28624 staining Human VIP Receptor 2 in human neuroendocrine cells of the small intestine by immunohistochemistry using paraffin embedded tissue.

  • ICC/IF image of ab28624 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28624, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-VPAC2 antibody (ab28624)

This product has been referenced in:
  • Bertolini M  et al. Vasoactive intestinal peptide, whose receptor-mediated signalling may be defective in alopecia areata, provides protection from hair follicle immune privilege collapse. Br J Dermatol 175:531-41 (2016). Read more (PubMed: 27059672) »
  • Miller JE  et al. Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian olfactory bulb and olfaction. J Neurosci 34:6040-6 (2014). Read more (PubMed: 24760863) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Rat Cell (Suprachiamatic Nucleus)
Specification Suprachiamatic Nucleus
Permeabilization Yes - Triton-X
Fixative Formaldehyde
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Verified customer

Submitted Aug 27 2013

Thank you for your inquiry.


I am double-checking with the lab about ab16155, but I can tell you that for ab28624 the protocol used citrate buffer:


Briefly, sections were dewaxed, microwaved in 10 mmol/L citric acid (pH 6...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"