• Product nameAnti-VPAC2 antibody
    See all VPAC2 primary antibodies
  • Description
    Rabbit polyclonal to VPAC2
  • Tested applicationsSuitable for: ICC/IF, ICC, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 419-438 of Human VIP Receptor 2

  • Positive control
    • Membrane preparations for HEK 293 cells stably expressing VPAC2.



Our Abpromise guarantee covers the use of ab28624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ICC 1/1000.
IHC-P 1/500.
WB 1/1000. Detects a band of approximately 50-60 kDa.


  • FunctionThis is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.
  • Tissue specificityExpressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Helodermin-preferring VIP receptor antibody
    • PACAP type III receptor antibody
    • PACAP-R-3 antibody
    • PACAP-R3 antibody
    • Pituitary adenylate cyclase-activating polypeptide type III receptor antibody
    • Vasoactive intestinal polypeptide receptor 2 antibody
    • VIP Receptor 2 antibody
    • VIP-R-2 antibody
    • Vipr2 antibody
    • VIPR2_HUMAN antibody
    • VPAC2 receptor antibody
    see all

Anti-VPAC2 antibody images

  • ab28624 staining Human VIP Receptor 2 in human neuroendocrine cells of the small intestine by immunohistochemistry using paraffin embedded tissue.

  • ICC/IF image of ab28624 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28624, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-VPAC2 antibody (ab28624)

This product has been referenced in:
  • Bertolini M  et al. Vasoactive intestinal peptide, whose receptor-mediated signalling may be defective in alopecia areata, provides protection from hair follicle immune privilege collapse. Br J Dermatol 175:531-41 (2016). Read more (PubMed: 27059672) »
  • Miller JE  et al. Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian olfactory bulb and olfaction. J Neurosci 34:6040-6 (2014). Read more (PubMed: 24760863) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Rat Cell (Suprachiamatic Nucleus)
Specification Suprachiamatic Nucleus
Permeabilization Yes - Triton-X
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Aug 27 2013

Thank you for your inquiry.

I am double-checking with the lab about ab16155, but I can tell you that for ab28624 the protocol used citrate buffer:

Briefly, sections were dewaxed, microwaved in 10 mmol/L citric acid (pH 6...

Read More