Overview

  • Product name
    Anti-VPH1 antibody [10D7A7B2]
  • Description
    Mouse monoclonal [10D7A7B2] to VPH1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified) corresponding to VPH1. Purified S. cerevisiae vacuole membranes

  • General notes

    This antibody clone is manufactured by Abcam.

    This IgG2a kappa isotype control monoclonal antibody was raised in mouse against a yeast-specific protein, V-ATPase subunit (the product of VPH1 gene). It is a suitable isotype control for mammalian species. 

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab113683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ICC/IF Use a concentration of 20 µg/ml. Heat induced antigen retrieval recommended.

Target

  • Function
    Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
  • Sequence similarities
    Belongs to the V-ATPase 116 kDa subunit family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Vacuole membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • V-ATPase 95 kDa subunit antibody
      • V-ATPase a 1 subunit antibody
      • V-type proton ATPase subunit a antibody
      • vacuolar isoform antibody
      • Vacuolar pH protein 1 antibody
      • Vacuolar proton pump a subunit antibody
      • Vacuolar proton translocating ATPase subunit a 1 antibody
      • vph1 antibody
      • VPH1_YEAST antibody
      see all

    Images

    • All lanes : Anti-VPH1 antibody [10D7A7B2] (ab113683)

      Lane 1 : Yeast whole cell lysate
      Lane 2 : yeast membrane preparation

      Lysates/proteins at 15 µg per lane.

      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 95 kDa

    References

    This product has been referenced in:
    • Dodgson SE  et al. The pleiotropic deubiquitinase Ubp3 confers aneuploidy tolerance. Genes Dev 30:2259-2271 (2016). Read more (PubMed: 27807036) »
    • Simpkins JA  et al. Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration. Biol Open 5:689-97 (2016). Read more (PubMed: 27142334) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (whole cell lysate)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    40 µg
    Specification
    whole cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 21 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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