Overview

  • Product nameAnti-VPH1 antibody [10D7A7B2]
  • Description
    Mouse monoclonal [10D7A7B2] to VPH1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified)

  • Positive control
    • Yeast membranes
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab113683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ICC/IF Use a concentration of 20 µg/ml. Heat induced antigen retrieval recommended.

Target

  • FunctionSubunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
  • Sequence similaritiesBelongs to the V-ATPase 116 kDa subunit family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationVacuole membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • V-ATPase 95 kDa subunit antibody
      • V-ATPase a 1 subunit antibody
      • V-type proton ATPase subunit a antibody
      • vacuolar isoform antibody
      • Vacuolar pH protein 1 antibody
      • Vacuolar proton pump a subunit antibody
      • Vacuolar proton translocating ATPase subunit a 1 antibody
      • vph1 antibody
      • VPH1_YEAST antibody
      see all

    Anti-VPH1 antibody [10D7A7B2] images

    • All lanes : Anti-VPH1 antibody [10D7A7B2] (ab113683)

      Lane 1 : Yeast whole cell lysate
      Lane 2 : yeast membrane preparation

      Lysates/proteins at 15 µg per lane.

      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 95 kDa

    References for Anti-VPH1 antibody [10D7A7B2] (ab113683)

    ab113683 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab113683.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"