Anti-VPS11 antibody (ab125083)
Key features and details
- Rabbit polyclonal to VPS11
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VPS11 antibody
See all VPS11 primary antibodies -
Description
Rabbit polyclonal to VPS11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 891-941 of Human VPS11 (NP_068375.3).
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Positive control
- 293T, Jurkat and HeLa whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab125083 was affinity purified using an epitope specific to VPS11 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 108 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. -
Tissue specificity
Ubiquitous. Expression was highest in heart and low in lung. -
Sequence similarities
Belongs to the VPS11 family.
Contains 1 clathrin repeat.
Contains 1 RING-type zinc finger. -
Cellular localization
Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle. - Information by UniProt
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Database links
- Entrez Gene: 489374 Dog
- Entrez Gene: 55823 Human
- Entrez Gene: 71732 Mouse
- Entrez Gene: 315600 Rat
- Omim: 608549 Human
- SwissProt: Q9H270 Human
- SwissProt: Q91W86 Mouse
- Unigene: 234282 Human
see all -
Alternative names
- END1 antibody
- HGNC:14583 antibody
- hVPS11 antibody
see all
Images
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All lanes : Anti-VPS11 antibody (ab125083) at 0.04 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 3 minutes -
Detection of VPS11 by Western Blot of Immunprecipitate.
ab125083 at 0.4µg/ml staining VPS11 in 293T whole cell lysate immunoprecipitated using ab125083 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab125083 has been referenced in 4 publications.
- Jongsma ML et al. SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport. EMBO J 39:e102301 (2020). PubMed: 32080880
- Marwaha R et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). PubMed: 28325809
- Sindhwani A et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). PubMed: 29084291
- Khatter D et al. The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes. J Cell Sci 128:1746-61 (2015). PubMed: 25908847