• Product name
  • Description
    Rabbit polyclonal to VPS11
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 891-941 of Human VPS11 (NP_068375.3).

  • Positive control
    • 293T, Jurkat and HeLa whole cell lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab125083 was affinity purified using an epitope specific to VPS11 immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab125083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
IP Use at 2-10 µg/mg of lysate.


  • Function
    May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
  • Tissue specificity
    Ubiquitous. Expression was highest in heart and low in lung.
  • Sequence similarities
    Belongs to the VPS11 family.
    Contains 1 clathrin repeat.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links
  • Alternative names
    • END1 antibody
    • HGNC:14583 antibody
    • hVPS11 antibody
    • PEP5 antibody
    • PP3476 antibody
    • RING finger protein 108 antibody
    • RNF108 antibody
    • Vacuolar protein sorting 11 (yeast homolog) antibody
    • Vacuolar protein sorting 11 (yeast) antibody
    • Vacuolar protein sorting 11 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 11 homolog antibody
    • Vacuolar protein sorting associated protein 11 homolog antibody
    • Vacuolar protein sorting protein 11 antibody
    • Vacuolar protein sorting-associated protein 11 homolog antibody
    • vps11 antibody
    • VPS11_HUMAN antibody
    see all

Anti-VPS11 antibody images

  • All lanes : Anti-VPS11 antibody (ab125083) at 0.04 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 108 kDa

    Exposure time : 3 minutes
  • Detection of VPS11 by Western Blot of Immunprecipitate.
    ab125083 at 0.4µg/ml staining VPS11 in 293T whole cell lysate immunoprecipitated using ab125083 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-VPS11 antibody (ab125083)

This product has been referenced in:
  • Marwaha R  et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). Read more (PubMed: 28325809) »
  • Khatter D  et al. The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes. J Cell Sci 128:1746-61 (2015). Read more (PubMed: 25908847) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab125083.
Please use the links above to contact us or submit feedback about this product.


Sign up