For the best experience on the Abcam website please upgrade to a modern browser such as
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab125083 was affinity purified using an epitope specific to VPS11 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
Use at 2-10 µg/mg of lysate.
Function May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Tissue specificity Ubiquitous. Expression was highest in heart and low in lung.
Sequence similarities Belongs to the VPS11 family. Contains 1 clathrin repeat. Contains 1 RING-type zinc finger.
Cellular localization Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
Information by UniProt
Anti-VPS11 antibody images
Western blot - Anti-VPS11 antibody (ab125083)
All lanes : Anti-VPS11 antibody (ab125083) at 0.04 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 50 µg Lane 4 : HeLa whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 108 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-VPS11 antibody (ab125083)
Detection of VPS11 by Western Blot of Immunprecipitate.
ab125083 at 0.4µg/ml staining VPS11 in 293T whole cell lysate immunoprecipitated using ab125083 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"