Overview

  • Product nameAnti-VPS11 antibody
    See all VPS11 primary antibodies
  • Description
    Rabbit polyclonal to VPS11
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 891-941 of Human VPS11 (NP_068375.3).

  • Positive control
    • 293T, Jurkat and HeLa whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab125083 was affinity purified using an epitope specific to VPS11 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionMay play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
  • Tissue specificityUbiquitous. Expression was highest in heart and low in lung.
  • Sequence similaritiesBelongs to the VPS11 family.
    Contains 1 clathrin repeat.
    Contains 1 RING-type zinc finger.
  • Cellular localizationLate endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links
  • Alternative names
    • END1 antibody
    • HGNC:14583 antibody
    • hVPS11 antibody
    • PEP5 antibody
    • PP3476 antibody
    • RING finger protein 108 antibody
    • RNF108 antibody
    • Vacuolar protein sorting 11 (yeast homolog) antibody
    • Vacuolar protein sorting 11 (yeast) antibody
    • Vacuolar protein sorting 11 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 11 homolog antibody
    • Vacuolar protein sorting associated protein 11 homolog antibody
    • Vacuolar protein sorting protein 11 antibody
    • Vacuolar protein sorting-associated protein 11 homolog antibody
    • vps11 antibody
    • VPS11_HUMAN antibody
    see all

Anti-VPS11 antibody images

  • All lanes : Anti-VPS11 antibody (ab125083) at 0.04 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 108 kDa


    Exposure time : 3 minutes
  • Detection of VPS11 by Western Blot of Immunprecipitate.
    ab125083 at 0.4µg/ml staining VPS11 in 293T whole cell lysate immunoprecipitated using ab125083 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-VPS11 antibody (ab125083)

This product has been referenced in:
  • Khatter D  et al. The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes. J Cell Sci 128:1746-61 (2015). Read more (PubMed: 25908847) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"