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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab125083 was affinity purified using an epitope specific to VPS11 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
Use at 2-10 µg/mg of lysate.
Function May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Tissue specificity Ubiquitous. Expression was highest in heart and low in lung.
Sequence similarities Belongs to the VPS11 family. Contains 1 clathrin repeat. Contains 1 RING-type zinc finger.
Cellular localization Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
Information by UniProt
Anti-VPS11 antibody images
Western blot - Anti-VPS11 antibody (ab125083)
All lanes : Anti-VPS11 antibody (ab125083) at 0.04 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 50 µg Lane 4 : HeLa whole cell lysate at 50 µg developed using the ECL technique Predicted band size : 108 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-VPS11 antibody (ab125083)
Detection of VPS11 by Western Blot of Immunprecipitate.
ab125083 at 0.4µg/ml staining VPS11 in 293T whole cell lysate immunoprecipitated using ab125083 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
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