Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 108 kDa. PubMed: 23724125

Target

  • Function
    May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
  • Tissue specificity
    Ubiquitous. Expression was highest in heart and low in lung.
  • Sequence similarities
    Belongs to the VPS11 family.
    Contains 1 clathrin repeat.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links
  • Alternative names
    • END1 antibody
    • HGNC:14583 antibody
    • hVPS11 antibody
    • PEP5 antibody
    • PP3476 antibody
    • RING finger protein 108 antibody
    • RNF108 antibody
    • Vacuolar protein sorting 11 (yeast homolog) antibody
    • Vacuolar protein sorting 11 (yeast) antibody
    • Vacuolar protein sorting 11 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 11 homolog antibody
    • Vacuolar protein sorting associated protein 11 homolog antibody
    • Vacuolar protein sorting protein 11 antibody
    • Vacuolar protein sorting-associated protein 11 homolog antibody
    • vps11 antibody
    • VPS11_HUMAN antibody
    see all

Images

  • ab40816 (3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

This product has been referenced in:
  • Clancey LF  et al. Maintenance of melanophore morphology and survival is cathepsin and vps11 dependent in zebrafish. PLoS One 8:e65096 (2013). WB ; Zebrafish . Read more (PubMed: 23724125) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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