Validated using a knockout cell line
Recombinant
RabMAb

Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)

Overview

  • Product name
    Anti-VPS26 antibody [EPR13455(B)] - N-terminal
    See all VPS26 primary antibodies
  • Description
    Rabbit monoclonal [EPR13455(B)] to VPS26 - N-terminal
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS26 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: O75436

  • Positive control
    • HepG2, HeLa and 293T cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab180172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
    • Sequence similarities
      Belongs to the VPS26 family.
    • Cellular localization
      Cytoplasm. Endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ12930 antibody
      • HB 58 antibody
      • HB58 antibody
      • Hbeta 58 antibody
      • Hbeta 58 homolog antibody
      • Hbeta58 antibody
      • Hbeta58 homolog antibody
      • hVPS 26 antibody
      • hVPS26 antibody
      • MGC94168 antibody
      • PEP 8A antibody
      • PEP8A antibody
      • Vacuolar protein sorting 26 A antibody
      • Vacuolar protein sorting 26 antibody
      • Vacuolar protein sorting 26 homolog A antibody
      • Vacuolar protein sorting 26 homolog A S. pombe antibody
      • Vacuolar protein sorting 26 homolog A yeast antibody
      • Vacuolar protein sorting 26 yeast antibody
      • Vacuolar protein sorting 26 yeast homolog antibody
      • Vacuolar protein sorting 26A antibody
      • Vacuolar protein sorting associated protein 26A antibody
      • Vacuolar protein sorting-associated protein 26A antibody
      • Vesicle protein sorting 26 antibody
      • Vesicle protein sorting 26A antibody
      • VP26A_HUMAN antibody
      • VPS 26 antibody
      • VPS 26A antibody
      • VPS26A antibody
      see all

    Images



    • Predicted band size : 38 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab180172 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab180172 was shown to specifically react with VPS26 when VPS26 knockout samples were used. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab180172 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labeled goat anti-rabbit at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 38 kDa

    References

    ab180172 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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