Overview

  • Product name
  • Description
    Goat polyclonal to VPS28
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide: ESAYNAFNRFLHA, corresponding to C terminal amino acids 209-211 of Human VPS28.

  • Positive control
    • Human testis and brain lysates.
  • General notes


    This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).Can be blocked with Human VPS28 peptide (ab23183).

Target

  • Function
    Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
  • Sequence similarities
    Belongs to the VPS28 family.
    Contains 1 VPS28 C-terminal domain.
    Contains 1 VPS28 N-terminal domain.
  • Cellular localization
    Cell membrane. Late endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESCRT-I complex subunit VPS28 antibody
    • H-Vps28 antibody
    • Vacuolar protein sorting 28 antibody
    • Vacuolar protein sorting 28 (yeast) antibody
    • Vacuolar protein sorting 28 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 28 homolog antibody
    • Vacuolar protein sorting-associated protein 28 homolog antibody
    • VPS 28 antibody
    • Vps28 antibody
    • VPS28_HUMAN antibody
    • Yeast class E protein Vps28p homolog antibody
    see all

Images



  • Predicted band size : 25 kDa


    ab10133 at 1µg/ml staining approximately 24kDa band of human VPS28 in human testis lysate by Western blot (ECL).
  • ab10133 (10µg/ml) staining of Human Testis by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer pH9 and HRP-staining.

References

This product has been referenced in:
  • Mikula M  et al. Genome-wide co-localization of active EGFR and downstream ERK pathway kinases mirrors mitogen-inducible RNA polymerase 2 genomic occupancy. Nucleic Acids Res 44:10150-10164 (2016). Read more (PubMed: 27587583) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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