Recombinant
RabMAb

Anti-VPS28 antibody [EPR9881(B)] (ab167172)

Overview

  • Product name
    Anti-VPS28 antibody [EPR9881(B)]
    See all VPS28 primary antibodies
  • Description
    Rabbit monoclonal [EPR9881(B)] to VPS28
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human VPS28 (UniProt: Q9UK41).

  • Positive control
    • HepG2, Jurkat, Human fetal brain, A549 and Human fetal liver cell lysates; Human kidney tissue; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-VPS28 antibody [EPR9881(B)] (ab167172) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : Human fetal brain lysates
      Lane 4 : A549 cell lysates
      Lane 5 : Human fetal liver lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 25 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS28 with ab167172 at 1/50 dilution.
    • Immunofluorescence analysis of HepG2 cells labeling VPS28 with ab167172 at 1/100 dilution.

    References

    ab167172 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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