Anti-VPS29 antibody (ab10160)
Key features and details
- Goat polyclonal to VPS29
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VPS29 antibody
See all VPS29 primary antibodies -
Description
Goat polyclonal to VPS29 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
Synthetic peptide: DDVKVERIEYKKP, corresponding to C terminal amino acids 170-182 of Human VPS29.
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Positive control
- Human muscle and kidney extracts.
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General notes
VPS29, VPS30, and VPS35 proteins are required for the normal recycling of Vps10p, a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
VPS29, VPS30, and VPS35 proteins are required for the normal recycling of Vps10p, a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 20 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 20 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). -
Tissue specificity
Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain. -
Sequence similarities
Belongs to the VPS29 family. -
Cellular localization
Cytoplasm. Membrane. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 477477 Dog
- Entrez Gene: 51699 Human
- Entrez Gene: 56433 Mouse
- Entrez Gene: 288666 Rat
- Omim: 606932 Human
- SwissProt: Q9UBQ0 Human
- SwissProt: Q9QZ88 Mouse
- SwissProt: B2RZ78 Rat
see all -
Alternative names
- DC15 antibody
- DC7 antibody
- DKFZp564F0223 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (9)
ab10160 has been referenced in 9 publications.
- Sargent D et al. Neuronal VPS35 deletion induces spinal cord motor neuron degeneration and early post-natal lethality. Brain Commun 3:fcab208 (2021). PubMed: 34704029
- Li JG et al. A pharmacological chaperone improves memory by reducing Aß and tau neuropathology in a mouse model with plaques and tangles. Mol Neurodegener 15:1 (2020). PubMed: 31964406
- Crawley-Snowdon H et al. Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29. Nat Commun 11:5031 (2020). PubMed: 33024112
- Rahman AA et al. Enhanced Hyaluronan Signaling and Autophagy Dysfunction by VPS35 D620N. Neuroscience 441:33-45 (2020). PubMed: 32540366
- Chen X et al. Parkinson's disease-linked D620N VPS35 knockin mice manifest tau neuropathology and dopaminergic neurodegeneration. Proc Natl Acad Sci U S A 116:5765-5774 (2019). PubMed: 30842285
- Li JG et al. Full recovery of the Alzheimer's disease phenotype by gain of function of vacuolar protein sorting 35. Mol Psychiatry N/A:N/A (2019). PubMed: 30733594
- Williams ET et al. Parkin mediates the ubiquitination of VPS35 and modulates retromer-dependent endosomal sorting. Hum Mol Genet 27:3189-3205 (2018). PubMed: 29893854
- Singh V et al. Cholera toxin inhibits SNX27-retromer-mediated delivery of cargo proteins to the plasma membrane. J Cell Sci 131:N/A (2018). PubMed: 30030371
- Zavodszky E et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun 5:3828 (2014). WB ; Human . PubMed: 24819384