Overview

  • Product name
  • Description
    Goat polyclonal to VPS29
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide: DDVKVERIEYKKP, corresponding to C terminal amino acids 170-182 of Human VPS29.

  • Positive control
    • Human muscle and kidney extracts.
  • General notes


    VPS29, VPS30, and VPS35 proteins are required for the normal recycling of Vps10p, a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    VPS29, VPS30, and VPS35 proteins are required for the normal recycling of Vps10p, a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 20 kDa.Can be blocked with Human VPS29 peptide (ab23191).

Target

  • Function
    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).
  • Tissue specificity
    Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.
  • Sequence similarities
    Belongs to the VPS29 family.
  • Cellular localization
    Cytoplasm. Membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DC15 antibody
    • DC7 antibody
    • DKFZp564F0223 antibody
    • FLJ20492 antibody
    • hVPS29 antibody
    • PEP11 antibody
    • PEP11 homolog antibody
    • Retromer protein antibody
    • Vacuolar protein sorting 29 antibody
    • Vacuolar protein sorting 29 (yeast homolog) antibody
    • Vacuolar protein sorting 29 (yeast) antibody
    • Vacuolar protein sorting 29 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 29 homolog antibody
    • Vacuolar protein sorting associated protein 29 antibody
    • Vacuolar protein sorting-associated protein 29 antibody
    • Vacuolar sorting protein VPS29/PEP11 antibody
    • Vesicle protein sorting 29 antibody
    • VPS 29 antibody
    • VPS29 antibody
    • VPS29 retromer complex component antibody
    • VPS29_HUMAN antibody
    • x 007 protein antibody
    see all

Images

  • Anti-VPS29 antibody (ab10160) at 0.1 µg/ml + Human Spleen lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size : 20 kDa
    Observed band size : 20 kDa

References

This product has been referenced in:
  • Zavodszky E  et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun 5:3828 (2014). WB ; Human . Read more (PubMed: 24819384) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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