Overview

  • Product name
  • Description
    Goat polyclonal to VPS29
  • Specificity
    This antibody is expected to recognise both reported isoforms
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Dog, Human
  • Immunogen

    Peptide with sequence CTGNLCTKESYDYLK, from the internal region of the protein sequence

  • Positive control
    • Human spleen lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml. In paraffin embedded Human Uterus shows vesicular and membranous staining in the endometrial glands.
WB Use at an assay dependent dilution. Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3µg/ml antibody staining (expected size : 20.9kDa). The 60kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size but we would welcome any feedback.

Target

  • Function
    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).
  • Tissue specificity
    Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.
  • Sequence similarities
    Belongs to the VPS29 family.
  • Cellular localization
    Cytoplasm. Membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DC15 antibody
    • DC7 antibody
    • DKFZp564F0223 antibody
    • FLJ20492 antibody
    • hVPS29 antibody
    • PEP11 antibody
    • PEP11 homolog antibody
    • Retromer protein antibody
    • Vacuolar protein sorting 29 antibody
    • Vacuolar protein sorting 29 (yeast homolog) antibody
    • Vacuolar protein sorting 29 (yeast) antibody
    • Vacuolar protein sorting 29 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 29 homolog antibody
    • Vacuolar protein sorting associated protein 29 antibody
    • Vacuolar protein sorting-associated protein 29 antibody
    • Vacuolar sorting protein VPS29/PEP11 antibody
    • Vesicle protein sorting 29 antibody
    • VPS 29 antibody
    • VPS29 antibody
    • VPS29 retromer complex component antibody
    • VPS29_HUMAN antibody
    • x 007 protein antibody
    see all

Anti-VPS29 antibody images

  • ab51972 at 3.8 µg/ml staining VPS29 in Human uterus tissue. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody detected by AP-staining.

References for Anti-VPS29 antibody (ab51972)

ab51972 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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