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Western blot - VPS35 antibody (ab10099)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VPS35 antibody (ab10099)
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Product Name
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VPS35 antibody
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See all VPS35 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to VPS35
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Immunogen
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Synthetic peptide: SPESEGPIYEGLIL, corresponding to C terminal amino acids 783-796 of Human VPS35.
BLAST 'C-SPESEGPIYEGLIL' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1/16000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa observed in human brain and testis lysates (predicted molecular weight: 91 kDa according to NP_060676).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Brain lysate.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
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Relevance
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VPS35 belongs to a group of vacuolar protein sorting (VPS) proteins. It is an essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans Golgi network. VPS35 is also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VPS35 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VPS35 antibody images, references, products related to ab10099 and other tools.
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VPS35 antibody images:
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 Western blot - VPS35 antibody (ab10099)
Predicted band size : 91 kDa
ab10099 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VPS35 antibody (ab10099)
ab10099 staining Human Testis by IHC-P. Image A) shows cytoplasmic staining of epithelial cells of the epididymis of the rete testis and image B), some leydig cells. Microwaved antigen retrieval with A) Tris/EDTA buffer pH9 at 3µg/ml and B) Citrate buffer pH6 at 10ug/ml, HRP-staining.
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References for VPS35 antibody (ab10099)
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This product has been used in: (two most recent references)
Scott KL et al. GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. Nature 459:1085-90 (2009). ICC/IF; Human. PubMed: 19553991
Gomez TS & Billadeau DD A FAM21-containing WASH complex regulates retromer-dependent sorting. Dev Cell 17:699-711 (2009). WB; Human. PubMed: 19922874
See all 4 publication references for this product.
If you publish research using ab10099 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to VPS35
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VPS35 antibody - more information
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Customer reviews (feedback) regarding VPS35 antibody |
Customer FAQs regarding VPS35 antibody |
Protocols for VPS35 antibody |
Price and availability of products related to VPS35 antibody |
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VPS35 antibody - related products:
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VPS35 antibody - other tools:
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Contact Abcam with a Technical Enquiry about VPS35 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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