Overview

  • Product nameAnti-VPS36 antibody
    See all VPS36 primary antibodies
  • Description
    Rabbit polyclonal to VPS36
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 91-140 (SNKEPGPFQS SKNSYIRLSF KEHGQIEFYR RLSEEMTQRR WETVPVSQSL) of Mouse VPS36 (NP_081614).

  • Positive control
    • Mouse liver lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab125715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionComponent of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.
  • Sequence similaritiesBelongs to the VPS36 family.
    Contains 1 GLUE C-terminal domain.
    Contains 1 GLUE N-terminal domain.
  • DomainThe GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.
  • Cellular localizationCytoplasm. Endosome. Late endosome. Membrane. Nucleus. Colocalizes with ubiquitinated proteins on late endosomes. Recruited to the endosome membrane to participate in vesicle formation.
  • Information by UniProt
  • Database links
  • Alternative names
    • C13orf9 antibody
    • CGI 145 antibody
    • EAP45 antibody
    • ELL associated protein of 45 kDa antibody
    • ELL-associated protein of 45 kDa antibody
    • ESCRT-II complex subunit VPS36 antibody
    • Vacuolar protein sorting associated protein 36 antibody
    • Vacuolar protein-sorting-associated protein 36 antibody
    • Vps36 antibody
    • VPS36_HUMAN antibody
    see all

Anti-VPS36 antibody images

  • Anti-VPS36 antibody (ab125715) at 1 µg/ml + Mouse liver lysate at 10 µg

    Predicted band size : 44 kDa

References for Anti-VPS36 antibody (ab125715)

ab125715 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"