Overview

  • Product nameAnti-VPS36 antibody
    See all VPS36 primary antibodies
  • Description
    Mouse polyclonal to VPS36
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length VPS36 protein (Human)

  • Positive control
    • VPS36 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.
    • Sequence similaritiesBelongs to the VPS36 family.
      Contains 1 GLUE C-terminal domain.
      Contains 1 GLUE N-terminal domain.
    • DomainThe GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.
    • Cellular localizationCytoplasm. Endosome. Late endosome. Membrane. Nucleus. Colocalizes with ubiquitinated proteins on late endosomes. Recruited to the endosome membrane to participate in vesicle formation.
    • Information by UniProt
    • Database links
    • Alternative names
      • C13orf9 antibody
      • CGI 145 antibody
      • EAP45 antibody
      • ELL associated protein of 45 kDa antibody
      • ELL-associated protein of 45 kDa antibody
      • ESCRT-II complex subunit VPS36 antibody
      • Vacuolar protein sorting associated protein 36 antibody
      • Vacuolar protein-sorting-associated protein 36 antibody
      • Vps36 antibody
      • VPS36_HUMAN antibody
      see all

    Anti-VPS36 antibody images

    • All lanes : Anti-VPS36 antibody (ab67801) at 1/500 dilution

      Lane 1 : VPS36 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

    References for Anti-VPS36 antibody (ab67801)

    ab67801 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab67801.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"