The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.
Sequence similaritiesBelongs to the VPS36 family. Contains 1 GLUE C-terminal domain. Contains 1 GLUE N-terminal domain.
DomainThe GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.
Cellular localizationCytoplasm. Endosome. Late endosome. Membrane. Nucleus. Colocalizes with ubiquitinated proteins on late endosomes. Recruited to the endosome membrane to participate in vesicle formation.