Overview

  • Product nameAnti-VPS39 antibody
    See all VPS39 primary antibodies
  • Description
    Rabbit polyclonal to VPS39
  • Tested applicationsSuitable for: ICC, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, against an 18 amino acid sequence from near the C terminal of Human VPS39 (AAQ05978).

  • Positive control
    • Rat liver tissue lysate.
  • General notes


    At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab107570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionMay play a role in clustering and fusion of late endosomes and lysosomes.
  • Tissue specificityUbiquitous. Highly expressed in heart, skeletal muscle, kidney, pancreas, brain, placenta and lung.
  • Sequence similaritiesBelongs to the VAM6/VPS39 family.
    Contains 1 CNH domain.
  • Cellular localizationCytoplasm. Lysosome membrane. Late endosome membrane.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • A230065P22Rik antibody
    • AW743070 antibody
    • FLJ21681 antibody
    • FLJ46546 antibody
    • hVam6p antibody
    • KIAA0770 antibody
    • RP23-348L16.12 antibody
    • TLP antibody
    • TRAP1 like protein antibody
    • Vacuolar protein sorting 39 antibody
    • Vacuolar protein sorting 39 homolog antibody
    • VAM6 antibody
    • Vam6/Vps39 like antibody
    • Vam6/Vps39 like protein antibody
    • Vam6/Vps39-like protein antibody
    • VPS 39 antibody
    • Vps39 antibody
    • VPS39_HUMAN antibody
    see all

Anti-VPS39 antibody images

  • Immunofluorescence of VPS39 in mouse liver tissue with ab107570 at 20 ug/mL.
  • All lanes : Anti-VPS39 antibody (ab107570) at 0.5 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat liver tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 102 kDa
  • Immunohistochemistry of VPS39 in mouse liver tissue with ab107570 at 5 µg/ml.

References for Anti-VPS39 antibody (ab107570)

This product has been referenced in:
  • Jiang P  et al. The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol Biol Cell 25:1327-37 (2014). Read more (PubMed: 24554770) »
  • Zheng L  et al. Notch signaling from the endosome requires a conserved dileucine motif. Mol Biol Cell 24:297-307 (2013). Human . Read more (PubMed: 23171551) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 22°C
Sample Human Cell (Cultured Osteosarcoma Cells)
Specification Cultured Osteosarcoma Cells
Permeabilization Yes - TBST
Fixative Paraformaldehyde
Username

jonathan larochelle

Verified customer

Submitted Apr 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"