Overview

  • Product name
  • Description
    Mouse polyclonal to VPS39
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human VPS39 protein (NP_056104.2)

  • Positive control
    • VPS39 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 102 kDa).

Target

  • Function
    May play a role in clustering and fusion of late endosomes and lysosomes.
  • Tissue specificity
    Ubiquitous. Highly expressed in heart, skeletal muscle, kidney, pancreas, brain, placenta and lung.
  • Sequence similarities
    Belongs to the VAM6/VPS39 family.
    Contains 1 CNH domain.
  • Cellular localization
    Cytoplasm. Lysosome membrane. Late endosome membrane.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing.
  • Alternative names
    • A230065P22Rik antibody
    • AW743070 antibody
    • FLJ21681 antibody
    • FLJ46546 antibody
    • hVam6p antibody
    • KIAA0770 antibody
    • RP23-348L16.12 antibody
    • TLP antibody
    • TRAP1 like protein antibody
    • Vacuolar protein sorting 39 antibody
    • Vacuolar protein sorting 39 homolog antibody
    • VAM6 antibody
    • Vam6/Vps39 like antibody
    • Vam6/Vps39 like protein antibody
    • Vam6/Vps39-like protein antibody
    • VPS 39 antibody
    • Vps39 antibody
    • VPS39_HUMAN antibody
    see all

Images

  • All lanes : Anti-VPS39 antibody (ab69669) at 1/500 dilution

    Lane 1 : VPS39 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

    Predicted band size : 102 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Liu X  et al. Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus. J Biol Chem 286:17079-90 (2011). Read more (PubMed: 21454559) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (hela, 293)
Loading amount
5 µg
Specification
hela, 293
Gel Running Conditions
Non-reduced Denaturing (10% SDS running gel, 150V)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Submitted Jul 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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