Overview

  • Product name
  • Description
    Rabbit polyclonal to VPS41
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 226-461 of VPS41 (Uniprot ID: P49754).

  • Positive control
    • NT2D1, U-87 MG and MCF7 whole cell lysates; Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

  • Relevance
    VPS41 is required for vacuolar assembly and vacuolar traffic. Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. VSP41 is the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known.
  • Cellular localization
    Cytoplasmic face of coated pits and vesicles.
  • Database links
  • Alternative names
    • HVPS41 antibody
    • hVps41p antibody
    • HVSP41 antibody
    • S53 antibody
    • Vacuolar assembly protein 41 antibody
    • Vacuolar protein sorting 41 (yeast homolog) antibody
    • Vacuolar protein sorting 41 (yeast) antibody
    • Vacuolar protein sorting 41 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 41 homolog antibody
    • vacuolar protein sorting-associated protein 41 homolog antibody
    • VPS 41 antibody
    see all

Images

  • Anti-VPS41 antibody (ab153925) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg

    Predicted band size: 99 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling VPS41 with ab153925 at 1/500 dilution.

References

ab153925 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153925.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up