Recombinant
RabMAb

Anti-VPS41 antibody [EPR13268] (ab181078)

Overview

  • Product name
    Anti-VPS41 antibody [EPR13268]
    See all VPS41 primary antibodies
  • Description
    Rabbit monoclonal [EPR13268] to VPS41
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS41 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P49754

  • Positive control
    • HeLa and Human fetal brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13268
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

Target

  • Relevance
    VPS41 is required for vacuolar assembly and vacuolar traffic. Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. VSP41 is the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known.
  • Cellular localization
    Cytoplasmic face of coated pits and vesicles.
  • Database links
  • Alternative names
    • HVPS41 antibody
    • hVps41p antibody
    • HVSP41 antibody
    • S53 antibody
    • Vacuolar assembly protein 41 antibody
    • Vacuolar protein sorting 41 (yeast homolog) antibody
    • Vacuolar protein sorting 41 (yeast) antibody
    • Vacuolar protein sorting 41 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 41 homolog antibody
    • vacuolar protein sorting-associated protein 41 homolog antibody
    • VPS 41 antibody
    see all

Images

  • All lanes : Anti-VPS41 antibody [EPR13268] (ab181078) at 1/1000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 99 kDa

References

This product has been referenced in:
  • Marwaha R  et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). Read more (PubMed: 28325809) »
  • Sindhwani A  et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). Read more (PubMed: 29084291) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Cell lysate - other (PC12)
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Loading amount
16 µg
Specification
PC12
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Cedric Asensio

Verified customer

Submitted Feb 13 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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