The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 0.3 - 1.0 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.
Tissue specificityUbiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver.
Sequence similaritiesBelongs to the STXBP/unc-18/SEC1 family.
Cellular localizationGolgi apparatus membrane. Endosome membrane. Associated with Golgi/endosomal vesicles and the trans-Golgi network.