• Product nameAnti-VPS4a antibody
    See all VPS4a primary antibodies
  • Description
    Mouse polyclonal to VPS4a
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human VPS4a protein (NP_037377.1).

  • Positive control
    • VPS4a transfected 293T lysate.



Our Abpromise guarantee covers the use of ab69415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).


  • FunctionInvolved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the AAA ATPase family.
    Contains 1 MIT domain.
  • DomainThe MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
  • Cellular localizationPrevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
  • Information by UniProt
  • Database links
  • Alternative names
    • hVPS4 antibody
    • Protein SKD2 antibody
    • SKD1 antibody
    • SKD1 homolog antibody
    • SKD1A antibody
    • SKD2 antibody
    • Vacuolar protein sorting 4 homolog A antibody
    • Vacuolar protein sorting 4, yeast, homolog of, A antibody
    • Vacuolar protein sorting factor 4A antibody
    • Vacuolar protein sorting-associated protein 4A antibody
    • vacuolar sorting protein 4 antibody
    • VPS4 antibody
    • VPS4-1 antibody
    • Vps4a antibody
    • VPS4A_HUMAN antibody
    see all

Anti-VPS4a antibody images

  • All lanes : Anti-VPS4a antibody (ab69415) at 1/500 dilution

    Lane 1 : VPS4a transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-VPS4a antibody (ab69415)

ab69415 has not yet been referenced specifically in any publications.

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