ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
Belongs to the AAA ATPase family. Contains 1 MIT domain.
The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
Immunocytochemical staining of paraformaldehyde fixed Hela cells labeling VPS4a using ab180581 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 dilution. Cells were counterstained with DAPI (blue)
Immunocytochemical staining of paraformaldehyde fixed MCF-7 cells labeling VPS4a using ab180581 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor®t 488) at 1/200. Cells were counterstainined with DAPI (blue)
Immunohistochemical stainin gof paraffin-embedded Human testis tissue labeling VPS4a with ab180581 at 1/2000 dilution followed by prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG. Sample was counterstained with Hematoxylin.
Western blot - Anti-VPS4a [EPR14545(B)] antibody (ab180581)
All lanes : Anti-VPS4a antibody [EPR14545(B)] (ab180581) at 1/50000 dilution
Lane 1 : Fetal brain lysate Lane 2 : MCF-7 cell lysate Lane 3 : Hela cell lysate Lane 4 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated