Recombinant
RabMAb

Anti-VPS4a antibody [EPR14545(B)] (ab180581)

Overview

  • Product name
    Anti-VPS4a antibody [EPR14545(B)]
    See all VPS4a primary antibodies
  • Description
    Rabbit monoclonal [EPR14545(B)] to VPS4a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS4a aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UN37

  • Positive control
    • Fetal brain, MCF-7, Hela, U87-MG lysates, human testis tissue, MCF-7 cells, U87-MG cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
ICC/IF 1/500.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/2000.

Target

  • Function
    Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the AAA ATPase family.
    Contains 1 MIT domain.
  • Domain
    The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
  • Cellular localization
    Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
  • Information by UniProt
  • Database links
  • Alternative names
    • hVPS4 antibody
    • Protein SKD2 antibody
    • SKD1 antibody
    • SKD1 homolog antibody
    • SKD1A antibody
    • SKD2 antibody
    • Vacuolar protein sorting 4 homolog A antibody
    • Vacuolar protein sorting 4, yeast, homolog of, A antibody
    • Vacuolar protein sorting factor 4A antibody
    • Vacuolar protein sorting-associated protein 4A antibody
    • vacuolar sorting protein 4 antibody
    • VPS4 antibody
    • VPS4-1 antibody
    • Vps4a antibody
    • VPS4A_HUMAN antibody
    see all

Images

  • Flow cytometric analysis of paraformaldehyde fixed MCF-7 cells labeling VPS4a using ab180581 at 1/30 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunocytochemical staining of paraformaldehyde fixed Hela cells labeling VPS4a using ab180581 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 dilution.  Cells were counterstained with DAPI (blue)

  • Immunocytochemical staining of paraformaldehyde fixed MCF-7 cells labeling VPS4a using ab180581 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor®t 488) at 1/200.  Cells were counterstainined with DAPI (blue)

  • Immunohistochemical stainin gof paraffin-embedded Human testis tissue labeling VPS4a with ab180581 at 1/2000 dilution followed by prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG.  Sample was counterstained with Hematoxylin.

  • All lanes : Anti-VPS4a antibody [EPR14545(B)] (ab180581) at 1/50000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 49 kDa

References

ab180581 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
African Green Monkey Cell (Vero cell)
Specification
Vero cell
Permeabilization
Yes - TritonX100 al 0,1%, Tween20
Fixative
Paraformaldehyde
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Submitted Dec 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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