• Product name
  • Description
    Rabbit polyclonal to VPS4B
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 350 and 400 of human VPS4B (NP_004860.2).

  • Positive control
    • HeLa and 293T whole cell lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab86303 was affinity purified using an epitope specific to VPS4B immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab86303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 49 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the AAA ATPase family.
      Contains 1 MIT domain.
    • Domain
      The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • 8030489C12Rik antibody
      • Cell migration inducing 1 antibody
      • Cell migration-inducing gene 1 protein antibody
      • MGC116271 antibody
      • MIG1 antibody
      • Protein SKD1 antibody
      • Skd1 antibody
      • SKD1B antibody
      • Suppressor of K(+) transport growth defect 1 antibody
      • Suppressor of K+ transport defect 1 antibody
      • Vacuolar protein sorting 4 homolog B (S. cerevisiae) antibody
      • Vacuolar protein sorting 4 homolog B antibody
      • Vacuolar protein sorting 4b antibody
      • Vacuolar protein sorting-associated protein 4B antibody
      • Vacuolar protein sorting-associating protein 4B antibody
      • VPS4 2 antibody
      • VPS42 antibody
      • Vps4b antibody
      • VPS4B_HUMAN antibody
      see all


    • All lanes : Anti-VPS4B antibody (ab86303) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 49 kDa
      Observed band size: 49 kDa

      Exposure time: 1 minute
    • Detection of VPS4B by Western Blot of Immunprecipitate.
      ab86303, at 1 µg/ml, staining VPS4B in HeLa whole cell lysate immunoprecipitated using ab86303 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 10 seconds.


    This product has been referenced in:

    See 1 Publication for this product

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