Overview

  • Product nameAnti-VPS52 antibody
    See all VPS52 primary antibodies
  • Description
    Rabbit polyclonal to VPS52
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide corresponding to a region between C terminal amino acids 613-641 of Human VPS52 (NP_072047.4).

  • Positive control
    • WB: MDA-MB435 cell line lysates IHC-P: Human colon carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab108054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 82 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceVesicle mediated protein sorting is important for the distribution of intracellular molecules into distinct organelles. More than 40 vacuolar protein sorting (VPS) genes are involved in this process. VPS52 is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. VPS52 is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.
  • Cellular localizationGolgi apparatus; trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.
  • Database links
  • Alternative names
    • RP5 1033B10 antibody
    • ARE1 antibody
    • dJ1033B10.5 antibody
    • DKFZp547I194 antibody
    • RP5-1033B10 antibody
    • SAC2 antibody
    • SAC2 suppressor of actin mutations 2 like (yeast) antibody
    • SAC2 suppressor of actin mutations 2 like protein antibody
    • SACM2L antibody
    • Vacuolar protein sorting 52 (yeast) antibody
    • vacuolar protein sorting 52 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 52 homolog antibody
    see all

Anti-VPS52 antibody images

  • Anti-VPS52 antibody (ab108054) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 82 kDa
  • ab108054, at a 1/50 dilution, staining VPS52 in formalin fixed and paraffin embedded Human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-VPS52 antibody (ab108054)

ab108054 has not yet been referenced specifically in any publications.

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