Overview

  • Product nameAnti-VPS52 antibody
    See all VPS52 primary antibodies
  • Description
    Mouse polyclonal to VPS52
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    full length human VPS52 corresponding to amino acids 1-723 (NP_072047).

  • Positive control
    • VPS52 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).

Target

  • RelevanceVesicle mediated protein sorting is important for the distribution of intracellular molecules into distinct organelles. More than 40 vacuolar protein sorting (VPS) genes are involved in this process. VPS52 is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. VPS52 is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.
  • Cellular localizationGolgi apparatus; trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.
  • Database links
  • Alternative names
    • RP5 1033B10 antibody
    • ARE1 antibody
    • dJ1033B10.5 antibody
    • DKFZp547I194 antibody
    • RP5-1033B10 antibody
    • SAC2 antibody
    • SAC2 suppressor of actin mutations 2 like (yeast) antibody
    • SAC2 suppressor of actin mutations 2 like protein antibody
    • SACM2L antibody
    • Vacuolar protein sorting 52 (yeast) antibody
    • vacuolar protein sorting 52 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 52 homolog antibody
    see all

Anti-VPS52 antibody images

  • All lanes : Anti-VPS52 antibody (ab72812) at 1/500 dilution

    Lane 1 : VPS52 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 82 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References for Anti-VPS52 antibody (ab72812)

ab72812 has not yet been referenced specifically in any publications.

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