The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceVPS72 (Vacuolar protein sorting 72 homolog) is a nuclear protein which can bind DNA. It is a subunit of the TRRAP/TIP60 HAT complex, and associates with the SWI2/SNF2-related CBP activator protein (SRCAP) chromatin remodeling complex, suggesting a role as a transcriptional regulator or in chromatin modification and remodeling.