Overview

  • Product nameAnti-VR1 antibody
    See all VR1 primary antibodies
  • Description
    Rabbit polyclonal to VR1
  • Tested applicationsSuitable for: IHC (PFA fixed), IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Guinea pig
  • Immunogen

    Synthetic peptide:

    (C)EDAEVFKDSMAPGEK

    conjugated to KLH, corresponding to C terminal amino acids 825 - 839 of Mouse TRPV1

Properties

Applications

Our Abpromise guarantee covers the use of ab31895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) 1/1000.
IHC-P 1/1000.
IHC-Fr 1/400. PubMed: 19153713

Target

  • FunctionReceptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • Tissue specificityWidely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • DomainThe association domain (AD) is necessary for self-association.
  • Post-translational
    modifications
    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Capsaicin receptor antibody
    • DKFZp434K0220 antibody
    • osm 9 like TRP channel 1 antibody
    • Osm-9-like TRP channel 1 antibody
    • OTRPC1 antibody
    • Transient receptor potential cation channel subfamily V member 1 antibody
    • TRPV 1 antibody
    • Trpv1 antibody
    • TRPV1_HUMAN antibody
    • Vanilloid receptor 1 antibody
    • Vanilloid receptor subtype 1 antibody
    • VR 1 antibody
    • VR1 antibody
    see all

Anti-VR1 antibody images

  • ab31895 at 1/1000 demonstrating VR1 immunoreactivity in mouse dorsal root ganglion. Animals were perfusion fixed with 2% paraformaldehyde.
  • ab31895 staining mouse inferior olive using Cy3-conjugated donkey anti-rabbit secondary antibodies (red color) and DAPI (blue) as a nuclear counterstain.

References for Anti-VR1 antibody (ab31895)

This product has been referenced in:
  • Chen Y  et al. Possible Loss of GABAergic Inhibition in Mice With Induced Adenomyosis and Treatment With Epigallocatechin-3-Gallate Attenuates the Loss With Improved Hyperalgesia. Reprod Sci N/A:N/A (2014). Mouse . Read more (PubMed: 24492488) »
  • Koh WU  et al. Perineural pretreatment of bee venom attenuated the development of allodynia in the spinal nerve ligation injured neuropathic pain model; an experimental study. BMC Complement Altern Med 14:431 (2014). IHC-P ; Rat . Read more (PubMed: 25366818) »

See all 11 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (olfactory epithelium)
Loading amount 40 µg
Specification olfactory epithelium
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12.5%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Oct 05 2011

Thank you for contacting us. I understand your customer's concerns regarding the anti-VR1 antibody ab31895. Please be assured that the observed precipitation sometimes happens for whole antiserum antibodies and there is no problem with t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"