Overview

  • Product nameAnti-VR1 antibody
    See all VR1 primary antibodies
  • Description
    Rabbit polyclonal to VR1
  • SpecificityImmunohistochemical analysis of rat dorsal root ganglia indicates a good specificity for this serum. Cross reactivity: This antibody is known to react with human and rat. Other species have not been tested.
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    CGSLKPEDAEVFKDSMVPGEK

    conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 819-838 of Rat Vanilloid Receptor 1.

  • General notesThis antibody will stain a selected population of sensory neurons in the dorsal root ganglion. It is unsuitable for staining nerve fibres or terminals.

Properties

Applications

Our Abpromise guarantee covers the use of ab6166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. See Peier et al.

Target

  • FunctionReceptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • Tissue specificityWidely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • DomainThe association domain (AD) is necessary for self-association.
  • Post-translational
    modifications
    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Capsaicin receptor antibody
    • DKFZp434K0220 antibody
    • osm 9 like TRP channel 1 antibody
    • Osm-9-like TRP channel 1 antibody
    • OTRPC1 antibody
    • Transient receptor potential cation channel subfamily V member 1 antibody
    • TRPV 1 antibody
    • Trpv1 antibody
    • TRPV1_HUMAN antibody
    • Vanilloid receptor 1 antibody
    • Vanilloid receptor subtype 1 antibody
    • VR 1 antibody
    • VR1 antibody
    see all

References for Anti-VR1 antibody (ab6166)

This product has been referenced in:

See all 2 Publications for this product

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I am sorry to hear of your customer's troubles using ab6166. I have read the attached complaint report and it does appear the most recent vial of ab6166 is not working as expected. I would be happy to offer a repla...

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Thank you for your enquiry and interest. This antibody is not purified and as the datasheet indicates we sell it as whole antiserum. ab6166 is a polyclonal antibody and there is batch-to-batch variation; some bleed may contain higher levels of red bloo...

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This antibody was raised against the intra-cellular C-terminal.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"