Anti-VRK1 antibody [5D1] - N-terminal (ab171933)

Overview

  • Product name
    Anti-VRK1 antibody [5D1] - N-terminal
    See all VRK1 primary antibodies
  • Description
    Mouse monoclonal [5D1] to VRK1 - N-terminal
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human VRK1 aa 1-19 (N terminal) (Cysteine residue).
    Sequence:

    MPRVKAAQAGRQSSAKRHL-C


    Database link: Q99986

  • Positive control
    • HeLa, TIG-1 and U2OS cell extracts; interphase and metaphase HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab171933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IF 1/1000.
IHC-P Use at an assay dependent concentration.
WB 1/200 - 1/1000. Predicted molecular weight: 45 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • Function
    Serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.
  • Tissue specificity
    Widely expressed. Highly expressed in fetal liver, testis and thymus.
  • Involvement in disease
    Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA).
  • Sequence similarities
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.
  • Cellular localization
    Nucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC117401 antibody
    • MGC138280 antibody
    • MGC142070 antibody
    • PCH1 antibody
    • PCH1A antibody
    • Serine/threonine protein kinase VRK1 antibody
    • Serine/threonine-protein kinase VRK1 antibody
    • Vaccinia related kinase 1 antibody
    • Vaccinia virus B1R related kinase 1 antibody
    • Vaccinia-related kinase 1 antibody
    • VRK1 antibody
    • VRK1_HUMAN antibody
    see all

Images

  • Lanes 1 & 4 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/100 dilution
    Lanes 2 & 5 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilution
    Lanes 3 & 6 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/1000 dilution

    Lanes 1-3 : HeLa cell extract (5x10e4 cells)
    Lanes 4-6 : U2OS cell extract (5x10e4 cells)

    Secondary
    All lanes : Alexa488 goat anti-mouse IgG

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilution

    Lane 1 : Extracts from TIG1 (5x10e4) cells: Luciferase RNAi (control)
    Lane 2 : Extracts from TIG1 (5x10e4) cells: VRK1-1 RNAi
    Lane 3 : Extracts from TIG1 (5x10e4) cells: VRK1-2 RNAi

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilution

    Lane 1 : Extracts from HeLa (5x10e4) cells: Luciferase RNAi (control)
    Lane 2 : Extracts from HeLa (5x10e4) cells: VRK1-1 RNAi
    Lane 3 : Extracts from HeLa (5x10e4) cells: VRK1-2 RNAi

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • Immunofluorescent analysis of paraformaldehyde-fixed interphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

  • Immunofluorescent analysis of paraformaldehyde-fixed metaphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

  • Immunofluorescent analysis of methanol-fixed interphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

  • Immunofluorescent analysis of methanol-fixed metaphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

    Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

  • Immunofluorescent analysis of formaldehyde-fixed interphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

  • Immunofluorescent analysis of formaldehyde-fixed metaphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

    Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

  • Immunofluorescent analysis of methanol-fixed interphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

  • Immunofluorescent analysis of methanol-fixed metaphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

    Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

References

ab171933 has not yet been referenced specifically in any publications.

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