• Product nameAnti-VRK1 antibody
    See all VRK1 primary antibodies
  • Description
    Rabbit polyclonal to VRK1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the center region of human VRK1.

  • Positive control
    • Mouse lung tissue lysate and HL-60 cell lysate.



Our Abpromise guarantee covers the use of ab71686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).


  • FunctionSerine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.
  • Tissue specificityWidely expressed. Highly expressed in fetal liver, testis and thymus.
  • Involvement in diseaseDefects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA).
  • Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.
  • Cellular localizationNucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC117401 antibody
    • MGC138280 antibody
    • MGC142070 antibody
    • PCH1 antibody
    • PCH1A antibody
    • Serine/threonine protein kinase VRK1 antibody
    • Serine/threonine-protein kinase VRK1 antibody
    • Vaccinia related kinase 1 antibody
    • Vaccinia virus B1R related kinase 1 antibody
    • Vaccinia-related kinase 1 antibody
    • VRK1 antibody
    • VRK1_HUMAN antibody
    see all

Anti-VRK1 antibody images

  • All lanes : Anti-VRK1 antibody (ab71686)

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : Transiently transfected 293 cell lysate

    Lysates/proteins at 2 µg per lane.

    Predicted band size : 45 kDa
  • All lanes : Anti-VRK1 antibody (ab71686) at 1/100 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : HL-60 cell lysate

    Lysates/proteins at 12.5 µg per lane.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 15 kDa,25 kDa,27 kDa,30 kDa,50 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-VRK1 antibody (ab71686)

This product has been referenced in:
  • Zhuang X  et al. Dephosphorylation of barrier-to-autointegration factor by protein phosphatase 4 and its role in cell mitosis. J Biol Chem 289:1119-27 (2014). WB ; Human . Read more (PubMed: 24265311) »

See 1 Publication for this product

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