Overview

  • Product nameAnti-VRK1 antibody
    See all VRK1 primary antibodies
  • Description
    Mouse polyclonal to VRK1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human VRK1 protein (NP_003375.1, 1 a.a. ~ 396 a.a)

  • Positive control
    • Transfected 293T cell lysate, human pancreas tissue lysate and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab76864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml of the purified antibody.
    WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.
    • Tissue specificityWidely expressed. Highly expressed in fetal liver, testis and thymus.
    • Involvement in diseaseDefects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA).
    • Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.
    • Cellular localizationNucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC117401 antibody
      • MGC138280 antibody
      • MGC142070 antibody
      • PCH1 antibody
      • PCH1A antibody
      • Serine/threonine protein kinase VRK1 antibody
      • Serine/threonine-protein kinase VRK1 antibody
      • Vaccinia related kinase 1 antibody
      • Vaccinia virus B1R related kinase 1 antibody
      • Vaccinia-related kinase 1 antibody
      • VRK1 antibody
      • VRK1_HUMAN antibody
      see all

    Anti-VRK1 antibody images

    • Anti-VRK1 antibody (ab76864) at 1/500 dilution + human pancreas tissue lysate at 25 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 45 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-VRK1 antibody (ab76864) at 1/500 dilution

      Lane 1 : VRK1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 45 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • ab76864 at 10µg/ml staining VRK1 in Hela cells.

    References for Anti-VRK1 antibody (ab76864)

    ab76864 has not yet been referenced specifically in any publications.

    Product Wall

    Following up my previous message with the offer of the discount code: the antibody immunogen shares 87% with the mouse protein so we do expect cross-reaction, though our guarantee does not currently apply to mouse samples.

    DISCOUNT CODE: ****** Expiration date: January 20, 2012 I am very pleased to hear your customer would like to test ab76864 on mouse samples in western blotting. This code will give your customer one free primary antibody before the expiration...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"