Anti-VSIG4 antibody (ab56037)

Overview

  • Product nameAnti-VSIG4 antibody
    See all VSIG4 primary antibodies
  • Description
    Rabbit polyclonal to VSIG4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: SHYTCEVTWQ TPDGNQVVRD KITELRVQKL SVSKPTVTTG SGYGFTVPQG, corresponding to N terminal amino acids 109-158 of Human VSIG4

  • Positive control
    • Jurkat cell lyaste.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab56037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionPhagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.
  • Tissue specificityAbundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages.
  • Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complement receptor of the immunoglobulin superfamily antibody
    • CRIg antibody
    • Ig superfamily protein antibody
    • Protein Z39Ig antibody
    • UNQ317/PRO362 antibody
    • V set and immunoglobulin domain containing 4 antibody
    • V-set and immunoglobulin domain-containing protein 4 antibody
    • VSIG4 antibody
    • VSIG4_HUMAN antibody
    • Z39IG antibody
    see all

Anti-VSIG4 antibody images

  • Anti-VSIG4 antibody (ab56037) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

References for Anti-VSIG4 antibody (ab56037)

ab56037 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Human Cell (monocytes from PBMC, Jurkat, THP-1, HEK293)
Permeabilization No
Gating Strategy CD14+ monocytes from PBMC respective cell line (see above)
Specification monocytes from PBMC, Jurkat, THP-1, HEK293
Preparation Cell harvesting/tissue preparation method: Monocytes were stained within PBMC which was isolated by density gradient centrifugation of whole blood Jurkat, THP-1 and HEK293 cell line were harvested out of cell culture flasks and resuspended in FACS Azide buffer
Sample buffer: FACS Azide buffer was used consisting of PBS + 2 mM EDTA + 0,1% Natriumazid + 2% FCS
Fixation no fixation
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Verified customer

Submitted Apr 13 2015

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