Overview

  • Product nameAnti-VSV-G tag antibody
    See all VSV-G tag primary antibodies
  • Description
    Rabbit polyclonal to VSV-G tag
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with Vesicular stomatitis virus.
  • Immunogen

    Full length protein

  • General notes0.2 µ filtered

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 10 kDa.

Target

  • RelevanceVesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Anti-VSV-G tag antibody images

  • All lanes : Anti-VSV-G tag antibody (ab83196) at 1/1000 dilution

    Lane 1 : Human HEK293 cells transfected with pMD2.G (VSVG encoding construct) whole cell lysate (labelled +VSVG)
    Lane 2 : Human HEK293 mock transfected (labelled -VSVG)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit polyclonal HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 10 kDa


    Exposure time : 10 seconds

    Image is courtesy of an anonymous AbReview.

    See Abreview

References for Anti-VSV-G tag antibody (ab83196)

ab83196 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cell line)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 10 µg
Treatment Transfected with pMD2.G or mock
Specification HEK293 cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
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Submitted Sep 28 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293 cell line)
Permeabilization No
Specification HEK293 cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Sep 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"