1/200 - 1/1000. We recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein.
Use at an assay dependent concentration.
ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
Vesicular stomatitis virus glycoprotein tag antibody
VSV epitope tag antibody
VSV tag antibody
VSV-G epitope tag antibody
vsvg tag antibody
Immunocytochemistry/ Immunofluorescence - VSV-G tag antibody (Biotin) (ab34774)Image courtesy of an anonymous Abreview.
ab34774 staining VSV-G tag in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.025% Triton-X in PBS and then blocked using 5% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat polyclonal conjugated to Alexa Fluor® 488 (green) used undiluted.
Western blot - VSV-G tag antibody (Biotin) (ab34774)Image courtesy of an anonymous Abreview.
Both lanes are ab34774 at a 1/500 dilution plus 20µg of whole cell lysate prepared from HeLa cells transfected with VSV-G. A Streptavidin HRP conjugate was used at a 1/2000 dilution. Primary antibody incubated for 16 hours at 4°C.Blocking step performed using 5% milk for 1 hour at 23°C.
ELISA - VSV-G tag antibody (Biotin) (ab34774)This image is courtesy of an anonymous Abreview
ab34774 detecting the VSV-G tag in HeLa cells expressing VSV-G by direct ELISA. Samples were blocked with 5% BSA for 1 hour at 23°C and incubated with the primary antibody (1/3000 in blocking buffer) for 16 hours at 4°C. An undiluted streptavidin-alkaline phosphatase conjugate polyclonal was used as the secondary antibody.