Overview

  • Product nameAnti-VSV-G tag antibody (Biotin)
    See all VSV-G tag primary antibodies
  • Description
    Rabbit polyclonal to VSV-G tag (Biotin)
  • ConjugationBiotin
  • SpecificityThis antibody is directed against VSV-G protein.
  • Tested applicationsSuitable for: ELISA, WB, ICC/IF, Flow Cytmore details
  • Immunogen

    Synthetic peptide corresponding to VSV-G tag aa 501-511.
    Sequence:

    YTDIEMNRLGK

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/3000 - 1/12000.
WB 1/500 - 1/2000. Predicted molecular weight: 57.5 kDa.
ICC/IF 1/200 - 1/1000. We recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein.
Flow Cyt Use at an assay dependent concentration.

ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceVesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Anti-VSV-G tag antibody (Biotin) images

  • ab34774 staining VSV-G tag in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.025% Triton-X in PBS and then blocked using 5% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat polyclonal conjugated to Alexa Fluor® 488 (green) used undiluted.

    See Abreview


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57.5 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

  • ab34774 detecting the VSV-G tag in HeLa cells expressing VSV-G by direct ELISA. Samples were blocked with 5% BSA for 1 hour at 23°C and incubated with the primary antibody (1/3000 in blocking buffer) for 16 hours at 4°C. An undiluted streptavidin-alkaline phosphatase conjugate polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-VSV-G tag antibody (Biotin) (ab34774)

This product has been referenced in:
  • Wan Z  et al. Fangchinoline inhibits human immunodeficiency virus type 1 replication by interfering with gp160 proteolytic processing. PLoS One 7:e39225 (2012). WB . Read more (PubMed: 22720080) »
  • Tani H  et al. Replication-competent recombinant vesicular stomatitis virus encoding hepatitis C virus envelope proteins. J Virol 81:8601-12 (2007). WB . Read more (PubMed: 17553880) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3 Mouse Fibroblasts expressing VSV-G)
Specification NIH3T3 Mouse Fibroblasts expressing VSV-G
Fixative Formaldehyde
Permeabilization Yes - 0.025% Triton-X in TBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jan 12 2011

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Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 Mouse Fibroblasts transfected with VSV-G)
Loading amount 20 µg
Specification NIH 3T3 Mouse Fibroblasts transfected with VSV-G
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Dec 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (NIH 3T3 Mouse Fibroblasts)
Specification NIH 3T3 Mouse Fibroblasts
Preparation Cell harvesting/tissue preparation method: Trypsin/centrifugation
Sample buffer: PBS 5%FCS
Fixation Formaldehyde
Permeabilization Yes - 0.025% Triton-X in PBS
Gating Strategy Size gated NIH 3T3
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Submitted Sep 23 2010

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Application ELISA
Sample Human Purified protein (HeLa cells expressing vsv-g)
Specification HeLa cells expressing vsv-g
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Type Direct
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Submitted Sep 17 2010

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Application Flow Cytometry
Sample Human Cell (Hela cells transfected with full length vsv-g)
Specification Hela cells transfected with full length vsv-g
Preparation Cell harvesting/tissue preparation method: Trypsin/centrifugation
Sample buffer: PBS 5%FCS
Fixation Formaldehyde
Permeabilization Yes - 0.025% Triton-X in PBS
Gating Strategy Size gated HeLa
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Submitted Sep 17 2010

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Application Immunocytochemistry
Sample Human Cultured Cells (Hela cells transfected with full length vsv-g)
Specification Hela cells transfected with full length vsv-g
Fixative Paraformaldehyde
Permeabilization Yes - 0.025% Triton-X in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Sep 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela cells transfected with vsv-g)
Loading amount 20 µg
Specification Hela cells transfected with vsv-g
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Sep 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.025% Triton-X in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Sep 16 2010

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Application Flow Cytometry
Sample Mouse Cell (embryonic stem cell)
Specification embryonic stem cell
Preparation Cell harvesting/tissue preparation method: dissociation buffer
Sample buffer: 0.2% BSA, 0.1% sodium azide in PBS
Permeabilization No
Gating Strategy live population using forward and side scatter
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Submitted Jan 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"