Overview

  • Product nameAnti-VSV-G tag antibody [P5D4] (HRP)
    See all VSV-G tag primary antibodies
  • Description
    Mouse monoclonal [P5D4] to VSV-G tag (HRP)
  • ConjugationHRP
  • SpecificityThis antibody recognizes native and denatured forms of VSV-G tagged proteins.In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.

  • EpitopeThe antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.
  • Positive control
    • Recombinant VSV-G-LacZ tagged fusion protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 0.164% Sodium phosphate, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberP5D4
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 120 kDa.

Target

  • RelevanceVesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • Alternative names
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Anti-VSV-G tag antibody [P5D4] (HRP) images

  • All lanes : Anti-VSV-G tag antibody [P5D4] (HRP) (ab49610) at 1/1000 dilution

    Lane 1 : recombinant VSV-G-LacZ tagged fusion protein at 0.05 µg
    Lane 2 : recombinant VSV-G-LacZ tagged fusion protein at 0.025 µg
    Lane 3 : recombinant VSV-G-LacZ tagged fusion protein at 0.01 µg
    Lane 4 : recombinant VSV-G-LacZ tagged fusion protein at 0.005 µg


    Observed band size : 120 kDa (why is the actual band size different from the predicted?)

References for Anti-VSV-G tag antibody [P5D4] (HRP) (ab49610)

This product has been referenced in:
  • Vega B  et al. Technical Advance: Surface plasmon resonance-based analysis of CXCL12 binding using immobilized lentiviral particles. J Leukoc Biol : (2011). WB . Read more (PubMed: 21593136) »

See 1 Publication for this product

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We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for...

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