Anti-VSX1 antibody (ab105818)

Overview

  • Product name
    Anti-VSX1 antibody
  • Description
    Rabbit polyclonal to VSX1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 10 - 60 (RSRSRALAPG CPPTGSRLRS FAINDLLGLE ADLPTPAEPG LRSNSGDPAE) of Mouse VSX1 (NP_473409).

  • Positive control
    • Mouse spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 39 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Binds to the 37-bp core of the locus control region (LCR) of the red/green visual pigment gene cluster. May regulate the activity of the LCR and the cone opsin genes at earlier stages of development.
  • Tissue specificity
    In the adult eye, expressed in lens, iris, ciliary body, choroid, optical nerve head and, most strongly, in retina, but not expressed in sclera and cornea. According to PubMed 11978762, expressed in adult retina but not in lens and cornea. Within adult retina, found exclusively in the inner nuclear layer. Isoform 1, isoform 2, isoform 3 and isoform 4 expressed in adult retina, but not in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. Not expressed in thymus and spleen. Expressed in embryonic craniofacial tissue. Expressed in fetal (week 14) retina. Strongly expressed in neonatal retina (day 0), weakly in neonatal lens (day 0), choroid (day 0) and cornea (day 0, 4; month 9).
  • Involvement in disease
    Defects in VSX1 are a cause of posterior polymorphous corneal dystrophy type 1 (PPCD1) [MIM:122000]. PPCD1 is a slowly progressive hereditary disorder of the corneal endothelium that leads to a variable degree of visual impairment usually in adulthood.
    Defects in VSX1 are a cause of keratoconus type 1 (KTCN1) [MIM:148300]. Keratoconus type 1 is a frequent corneal dystrophy with an incidence that varies from 50 to 230 per 100'000. The cornea assumes a conical shape as a result of a progressive non-inflammatory thinning of the corneal stroma. Keratoconus is most often an isolated sporadic condition with cases of autosomal dominant and autosomal recessive transmission.
  • Sequence similarities
    Belongs to the paired homeobox family.
    Contains 1 CVC domain.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAASDS antibody
    • Homeodomain protein RINX antibody
    • KTCN antibody
    • KTCN1 antibody
    • Posterior polymorphous corneal dystrophy antibody
    • PPCD antibody
    • PPD antibody
    • Retinal inner nuclear layer homeobox protein antibody
    • RINX antibody
    • Transcription factor VSX1 antibody
    • Visual system homeobox 1 antibody
    • Visual system homeobox 1 homolog, CHX10 like antibody
    • Visual system homeobox 1 homolog, CHX10 like (zebrafish) antibody
    • VSX1 antibody
    • VSX1_HUMAN antibody
    see all

Images

  • Anti-VSX1 antibody (ab105818) (0.2 - 1 ug/ml) + Mouse spleen tissue lysate

    Predicted band size : 39 kDa

References

ab105818 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Embryonic spinal cord)
Specification
Embryonic spinal cord
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate
Permeabilization
Yes - 0.1% TritonX-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Frederic Clotman

Verified customer

Submitted Feb 06 2013

DISCOUNT CODE: xxxxx
Expiration date: xxxxxxxx
Value: €‘xxxxx

I am very pleased to hear you would like to test ab105818 in IHC-Fr. This code will give you €‘xxxxoff your next order before the expiration date. To redeem th...

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