Anti-VTA1 antibody (ab96738)
Key features and details
- Rabbit polyclonal to VTA1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VTA1 antibody -
Description
Rabbit polyclonal to VTA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a sequence within amino acids 1-246 of Human VTA1
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Positive control
- H1299, HeLaS3, Molt4 whole cell lysates; SW480 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (By similarity). Involved in HIV-1 budding. -
Sequence similarities
Belongs to the VTA1 family. -
Cellular localization
Cytoplasm. Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 506430 Cow
- Entrez Gene: 51534 Human
- Entrez Gene: 66201 Mouse
- Entrez Gene: 292640 Rat
- Omim: 610902 Human
- SwissProt: Q32L63 Cow
- SwissProt: Q9NP79 Human
- SwissProt: Q9CR26 Mouse
see all -
Alternative names
- C6orf55 antibody
- Chromosome 6 open reading frame 55 antibody
- Dopamine responsive protein DRG 1 antibody
see all
Images
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All lanes : Anti-VTA1 antibody (ab96738) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLaS3 whole cell lysate
Lane 3 : Molt4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
12% SDS-PAGE. -
ab96738 at 1/500 dilution staining VTA1 in SW480 xenograft by Immunohistochemistry, Paraffin-embedded tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab96738 has not yet been referenced specifically in any publications.