The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/32000 (detection limit).
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.