Overview

  • Product name
    VU 0360223
  • Description
    Potent mGlu5 antagonist
  • Biological description
    Negative allosteric mGlu5 modulator (IC50 = 61 nM). Inactive against mGlu1-4 and mGlu7-8. Centrally active and crosses the blood-brain barrier.
  • Purity
    > 98%

Properties

Applications

Our Abpromise guarantee covers the use of ab120803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab133477 staining GluR2 in SK-N-SH cells treated with VU 0360223 (ab120803), by ICC/IF. Decrease of GluR2 expression correlates with increased concentration of VU 0360223, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120803 (VU 0360223) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab133477 (1/200 dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Lindsley CW  et al. (3-Cyano-5-fluorophenyl)biaryl negative allosteric modulators of mGlu(5): Discovery of a new tool compound with activity in the OSS mouse model of addiction. ACS Chem Neurosci 2:471-482 (2011). Read more (PubMed: 21927650) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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