Overview

  • Product nameVU0155041 sodium salt
  • Description
    PAM for mGluR4; water soluble
  • Biological descriptionPositive allosteric selective modulator at mGluR4 (EC50 values are 798 and 693 nM at human and rat mGluR4 receptors, respectively). It has advantages over PHCCC (ab120043) as it is water soluble and more potent. Shows limited blood-brain barrier permeability. Active in behavioural models of Parkinson's disease following icv injection.

    Also available in simple stock solutions (ab146686) - add 1 ml of water to get an exact, ready-to-use concentration.
  • Purity> 98%

Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab120248 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    VU0155041 sodium salt images

    • SKNSH cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of VU0155041 sodium salt (ab120248). Increased expression of Gli1 (ab49314) in SKNSH cells correlates with an decrease in VU0155041 sodium salt concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 30 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab49314 at 2.5 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti- rabbit antibody conjugated to HRP (ab97051) at 1/10000 and visualised using ECL development solution.

    References for VU0155041 sodium salt (ab120248)

    This product has been referenced in:
    • Williams R  et al. Synthesis and SAR of a novel positive allosteric modulator (PAM) of the metabotropic glutamate receptor 4 (mGluR4). Bioorg Med Chem Lett 19:4967-70 (2009). Read more (PubMed: 19640716) »
    • Niswender CM  et al. Discovery, characterization, and antiparkinsonian effect of novel positive allosteric modulators of metabotropic glutamate receptor 4. Mol Pharmacol 74:1345-58 (2008). Read more (PubMed: 18664603) »

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"