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Rabbit polyclonal to VWA5B2
antigen sequence: PRKPSLGAIL DGPSPEPGQQ LGQGLDDSGN LLSPAPMDWD MLMEPPFLFT AVPPSGELAP PAVPPQAPRC HVVIRGLCGE QP, corresponding to amino acids 781-862 of Human VWA5B2 (UniProt ID: Q8N398).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 1 VIT domain.
Contains 1 VWFA domain.
- von Willebrand factor A domain containing 5B2 antibody
Immunocytochemistry/ Immunofluorescence - Anti-VWA5B2 antibody (ab122372)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, nucleoli and golgi apparatus. Recommended concentration of ab122372 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VWA5B2 antibody (ab122372)
ab122372, at a 1/200 dilution, staining VWA5B2 in paraffin embedded Human colon tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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