Overview

  • Product name
  • Description
    Rabbit polyclonal to WAPL
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1125 and 1175 of human WAPL (SwissProt entry Q7Z5K2)

  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab70741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/10000. Detects a band of approximately 175 kDa (predicted molecular weight: 133 kDa).

Target

  • Function
    Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
  • Tissue specificity
    Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
  • Sequence similarities
    Contains 1 WAPL domain.
  • Cellular localization
    Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • FOE antibody
    • Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
    • Friend of EBNA2 protein antibody
    • KIAA0261 antibody
    • OTTHUMP00000020004 antibody
    • WAPAL antibody
    • WAPL antibody
    • WAPL_HUMAN antibody
    • Wings apart like homolog antibody
    • Wings apart like homolog (Drosophila) antibody
    • Wings apart-like protein homolog antibody
    see all

Images

  • All lanes : Anti-WAPL antibody (ab70741) at 0.2 µg/ml

    Lane 1 : HeLa nuclear extract at 5 µg
    Lane 2 : HeLa nuclear extract at 20 µg


    Predicted band size : 133 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • All lanes : Anti-WAPL antibody (ab70741) at 1/2000 dilution

    Lane 1 : Human U2OS cells
    Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL.
    Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL.

    Lysates/proteins at 90 µg per lane.

    Secondary
    Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 133 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    See Abreview

References

This product has been referenced in:
  • Stelloh C  et al. The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics Chromatin 9:14 (2016). Read more (PubMed: 27087855) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
90 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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