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Rabbit monoclonal [EPR3407(2)] to WAPL
ICC/IF more details Unsuitable for:
Flow Cyt or IHC-P
A synthetic peptide corresponding to residues in Human WAPL.
HeLa, 293T, and Jurkat cell lysates. HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000 - 1/200000. Predicted molecular weight: 133 kDa.
1/10 - 1/100.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IHC-P.
Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
Contains 1 WAPL domain.
Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
Information by UniProt
Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
Friend of EBNA2 protein antibody
Western blot - WAPL antibody [EPR3407(2)] (ab109537)
All lanes : Anti-WAPL antibody [EPR3407(2)] (ab109537) at 1/50000 dilution Lane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lane 3 : Jurkat cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 133 kDa
Immunocytochemistry/ Immunofluorescence - WAPL antibody [EPR3407(2)] (ab109537)
ab109537 at 1/100 dilution staining WAPL in HeLa cells by Immunofluorescence.
Western blot - Anti-WAPL antibody [EPR3407(2)] (ab109537)
All lanes :
Anti-WAPL antibody [EPR3407(2)] (ab109537) at 1/10000 dilution
Lane 1 :
Human U2OS cells treated with siRNA control.
Lane 2 :
Human U2OS cells treated with siRNA #1 against WAPL.
Lane 3 :
Human U2OS cells treated with siRNA #2 against WAPL.
Lysates/proteins at 90 µg per lane.
Secondary All lanes :
Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
150 kDa (
why is the actual band size different from the predicted?
Additional bands at:
120 kDa (possible cross reactivity), 130 kDa (possible cross reactivity), 145 kDa (possible cross reactivity)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"