Anti-WAPL antibody [EPR3407(2)] (ab109537)

Overview

  • Product nameAnti-WAPL antibody [EPR3407(2)]
    See all WAPL primary antibodies
  • Description
    Rabbit monoclonal [EPR3407(2)] to WAPL
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human WAPL.

  • Positive control
    • HeLa, 293T, and Jurkat cell lysates. HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 133 kDa.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionRegulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
    • Tissue specificityIsoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
    • Sequence similaritiesContains 1 WAPL domain.
    • Cellular localizationNucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
    • Information by UniProt
    • Database links
    • Alternative names
      • FOE antibody
      • Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
      • Friend of EBNA2 protein antibody
      • KIAA0261 antibody
      • OTTHUMP00000020004 antibody
      • WAPAL antibody
      • WAPL antibody
      • WAPL_HUMAN antibody
      • Wings apart like homolog antibody
      • Wings apart like homolog (Drosophila) antibody
      • Wings apart-like protein homolog antibody
      see all

    Anti-WAPL antibody [EPR3407(2)] images

    • All lanes : Anti-WAPL antibody [EPR3407(2)] (ab109537) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 133 kDa
    • ab109537 at 1/100 dilution staining WAPL in HeLa cells by Immunofluorescence.
    • All lanes : Anti-WAPL antibody [EPR3407(2)] (ab109537) at 1/10000 dilution

      Lane 1 : Human U2OS cells treated with siRNA control.
      Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL.
      Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL.

      Lysates/proteins at 90 µg per lane.

      Secondary
      Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 133 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 120 kDa (possible cross reactivity),130 kDa (possible cross reactivity),145 kDa (possible cross reactivity).

      See Abreview

    References for Anti-WAPL antibody [EPR3407(2)] (ab109537)

    ab109537 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
    Loading amount 90 µg
    Specification Human osteosarcoma U2OS cells
    Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Verified customer

    Submitted Oct 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"