Overview

  • Product nameAnti-Warfarin antibody
  • Description
    Sheep polyclonal to Warfarin
  • Tested applicationsSuitable for: ELISA, Competitive ELISA, Purificationmore details
  • Immunogen

    Warfarin conjugated to Bovine thyroglobulin.

Properties

Applications

Our Abpromise guarantee covers the use of ab123975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 2.5 µg/ml.
Competitive ELISA Use a concentration of 2.5 µg/ml. 10ng/ml Warfarin produces 69% inhibition in a competitive ELISA employing ab123975
Purification Use a concentration of 2.5 µg/ml. ab123975 is suitable for the development of immunoaffinity purification columns.

Target

  • RelevanceWarfarin, a synthetic derivative of coumarinan, is an anticoagulant medication used for the prophylaxis of thrombosis and embolism in many disorders. It was originally developed as a rat poison, and is still widely used as such, although warfarin-resistant rats are becoming more common. Warfarin inhibits the effective synthesis of biologically active forms of the vitamin K-dependent clotting factors: II, VII, IX and X, as well as the regulatory factors protein C, protein S and protein Z. Other proteins not involved in blood clotting, such as osteocalcin, may also be affected. The precursors of these factors require carboxylation of their glutamic acid residues to allow the coagulation factors to bind to phospholipid surfaces. This carboxylation is linked to oxidation of vitamin K to form Vitamin K epoxide, which is in turn recycled back to the reduced form by the enzyme epoxide reductase. Warfarin inhibits epoxide reductase, thereby diminishing vitamin K action and inhibiting production of functioning coagulation factors. As the body's stores of previously-produced factors degrade (over several days), the anticoagulation effect becomes apparent. The coagulation factors are produced, but have decreased functionality due to undercarboxylation; they are collectively referred to as PIVKAs (proteins induced [by] vitamin K absence/antagonism).

References for Anti-Warfarin antibody (ab123975)

ab123975 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"