Overview

  • Product nameAnti-WASF3 antibody
    See all WASF3 primary antibodies
  • Description
    Mouse polyclonal to WASF3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-499 of Human WASF3

  • Positive control
    • Rat brain, transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDownstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.
    • Tissue specificityExpressed in ovary and brain.
    • Sequence similaritiesBelongs to the SCAR/WAVE family.
      Contains 1 WH2 domain.
    • DomainBinds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0900 antibody
      • Protein WAVE-3 antibody
      • Protein WAVE3 antibody
      • SCAR3 antibody
      • Verprolin homology domain containing protein 3 antibody
      • Verprolin homology domain-containing protein 3 antibody
      • WASF3 antibody
      • WASF3_HUMAN antibody
      • WASP family protein member 3 antibody
      • WAVE3 antibody
      • Wiskott Aldrich syndrome protein family member 3 antibody
      • Wiskott-Aldrich syndrome protein family member 3 antibody
      see all

    Anti-WASF3 antibody images

    • Anti-WASF3 antibody (ab68031) at 1/500 dilution + rat brain at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 55 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-WASF3 antibody (ab68031) at 1/500 dilution

      Lane 1 : WASF3 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 55 kDa
      Observed band size : 55 kDa
      Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-WASF3 antibody (ab68031)

    ab68031 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68031.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"