Overview

  • Product name
  • Description
    Goat polyclonal to WASP
  • Specificity
    This antibody reacts Wiskott-Aldrich Syndrome Protein. It is not expected to cross-react with N WASP (WASL).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    C-SPADKKRSGKK KI

    , corresponding to Internal sequence amino acids 220-232 of Human WASP

  • Positive control
    • U937 and MOLT4 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Detection limit 1/128,000,
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 53 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
    • Tissue specificity
      Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
    • Involvement in disease
      Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
      Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
      Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
    • Sequence similarities
      Contains 1 CRIB domain.
      Contains 1 WH1 domain.
      Contains 1 WH2 domain.
    • Domain
      The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
      The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Eczema thrombocytopenia antibody
      • IMD2 antibody
      • SCNX antibody
      • THC antibody
      • THC1 antibody
      • Thrombocytopenia 1 (X linked) antibody
      • U42471 antibody
      • Was antibody
      • WASp antibody
      • WASP_HUMAN antibody
      • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
      • Wiskott Aldrich syndrome antibody
      • Wiskott Aldrich syndrome protein antibody
      • Wiskott-Aldrich syndrome protein antibody
      see all

    Images

    • Anti-WASP antibody (ab28769) at 0.03 µg/ml + U937 cell lysate (total protein/per lane) at 30 µg/ml
      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 53-65 kDa (why is the actual band size different from the predicted?)

    References

    ab28769 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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