Overview

  • Product nameAnti-WASP antibody
    See all WASP primary antibodies
  • Description
    Rabbit polyclonal to WASP
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human WASP .

    (Peptide available as ab74903.)

  • Positive control
    • This antibody gave a positive signal in the following Whole Cell Lysates: Saos 2, LOVO, HT 1080, EBS E14 TG2A Day 0

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionEffector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
  • Tissue specificityExpressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
  • Involvement in diseaseDefects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
    Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
    Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
  • Sequence similaritiesContains 1 CRIB domain.
    Contains 1 WH1 domain.
    Contains 1 WH2 domain.
  • DomainThe WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
    The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Eczema thrombocytopenia antibody
    • IMD2 antibody
    • SCNX antibody
    • THC antibody
    • THC1 antibody
    • Thrombocytopenia 1 (X linked) antibody
    • U42471 antibody
    • Was antibody
    • WASp antibody
    • WASP_HUMAN antibody
    • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
    • Wiskott Aldrich syndrome antibody
    • Wiskott Aldrich syndrome protein antibody
    • Wiskott-Aldrich syndrome protein antibody
    see all

Anti-WASP antibody images

  • All lanes : Anti-WASP antibody (ab74904) at 1 µg/ml

    Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 4 : EBS E14 TG2A Day 0 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • IHC image of WASP staining in human Cerebral cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74904, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • All lanes : Anti-WASP antibody (ab74904) at 1 µg/ml

    Lane 1 : Saos-2
    Lane 2 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab74904 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74904, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-WASP antibody (ab74904)

ab74904 has not yet been referenced specifically in any publications.

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