Recombinant Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EPR2540Y] (ab68182)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2540Y] to WASP/Wiskott-Aldrich syndrome protein
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EPR2540Y]
See all WASP/Wiskott-Aldrich syndrome protein primary antibodies -
Description
Rabbit monoclonal [EPR2540Y] to WASP/Wiskott-Aldrich syndrome protein -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human WASP/Wiskott-Aldrich syndrome protein aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- TF1, U937 and Jurkat whole cell lysate (ab7899).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2540Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. -
Tissue specificity
Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen. -
Involvement in disease
Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia. -
Sequence similarities
Contains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 1 WH2 domain. -
Domain
The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7454 Human
- Entrez Gene: 22376 Mouse
- Entrez Gene: 317371 Rat
- Omim: 300392 Human
- SwissProt: P42768 Human
- SwissProt: P70315 Mouse
- Unigene: 2157 Human
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Alternative names
- Eczema thrombocytopenia antibody
- IMD2 antibody
- SCNX antibody
see all
Images
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All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EPR2540Y] (ab68182) at 1/2000 dilution
Lane 1 : TF1 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab68182 has been referenced in 2 publications.
- Wang F et al. EPHA2 Promotes the Invasion and Migration of Human Tongue Squamous Cell Carcinoma Cal-27 Cells by Enhancing AKT/mTOR Signaling Pathway. Biomed Res Int 2021:4219690 (2021). PubMed: 33834064
- Serafino A et al. Thymosin a1 modifies podosome architecture and promptly stimulates the expression of podosomal markers in mature macrophages. Expert Opin Biol Ther 15 Suppl 1:S101-16 (2015). PubMed: 26098689