Overview

  • Product nameAnti-WASP antibody [EPR2540Y]
    See all WASP primary antibodies
  • Description
    Rabbit monoclonal [EPR2540Y] to WASP
  • Tested applicationsSuitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues near the N terminus of human WASP

  • Positive control
    • TF1, U937 and Jurkat cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR2540Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionEffector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
    • Tissue specificityExpressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
    • Involvement in diseaseDefects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
      Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
      Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
    • Sequence similaritiesContains 1 CRIB domain.
      Contains 1 WH1 domain.
      Contains 1 WH2 domain.
    • DomainThe WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
      The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Eczema thrombocytopenia antibody
      • IMD2 antibody
      • SCNX antibody
      • THC antibody
      • THC1 antibody
      • Thrombocytopenia 1 (X linked) antibody
      • U42471 antibody
      • Was antibody
      • WASp antibody
      • WASP_HUMAN antibody
      • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
      • Wiskott Aldrich syndrome antibody
      • Wiskott Aldrich syndrome protein antibody
      • Wiskott-Aldrich syndrome protein antibody
      see all

    Anti-WASP antibody [EPR2540Y] images

    • All lanes : Anti-WASP antibody [EPR2540Y] (ab68182) at 1/2000 dilution

      Lane 1 : TF1 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 53 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    References for Anti-WASP antibody [EPR2540Y] (ab68182)

    ab68182 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68182.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"