Overview

  • Product name
    Anti-WASP (phospho Y290) antibody
    See all WASP primary antibodies
  • Description
    Rabbit polyclonal to WASP (phospho Y290)
  • Specificity
    ab59278 detects endogenous levels of WASP only when phosphorylated at tyrosine 290.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized phosphopeptide derived from human WASP around the phosphorylation site of tyrosine 290 (L-I-YP-D-F).

  • Positive control
    • WB: Extracts from HepG2 cells. IHC-P: Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Function
    Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
  • Tissue specificity
    Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
  • Involvement in disease
    Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
    Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
    Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
  • Sequence similarities
    Contains 1 CRIB domain.
    Contains 1 WH1 domain.
    Contains 1 WH2 domain.
  • Domain
    The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
    The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Eczema thrombocytopenia antibody
    • IMD2 antibody
    • SCNX antibody
    • THC antibody
    • THC1 antibody
    • Thrombocytopenia 1 (X linked) antibody
    • U42471 antibody
    • Was antibody
    • WASp antibody
    • WASP_HUMAN antibody
    • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
    • Wiskott Aldrich syndrome antibody
    • Wiskott Aldrich syndrome protein antibody
    • Wiskott-Aldrich syndrome protein antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59278 at a dilution of 1/50.
    Left image: no immunising peptide.
    Right hand image: with immunising peptide.
  • All lanes : Anti-WASP (phospho Y290) antibody (ab59278) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells with no immunising peptide
    Lane 2 : Extracts from HepG2 cells with immunising peptide


    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Liu C  et al. N-wasp is essential for the negative regulation of B cell receptor signaling. PLoS Biol 11:e1001704 (2013). Flow Cyt, ICC/IF ; Mouse, Human . Read more (PubMed: 24223520) »
  • Kumar S  et al. Cdc42 regulates neutrophil migration via crosstalk between WASp, CD11b, and microtubules. Blood 120:3563-74 (2012). Read more (PubMed: 22932798) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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