The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 0.2 - 0.4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa). Staining of the WAVE band is specifically
inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDownstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Tissue specificityHighly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
Sequence similaritiesBelongs to the SCAR/WAVE family. Contains 1 WH2 domain.
DomainBinds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
Cellular localizationCytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.
Payne LJ et al. p53 Down Regulates PDGF-Induced Formation of Circular Dorsal Ruffles in Rat Aortic Smooth Muscle Cells. PLoS One9:e108257 (2014).
Read more (PubMed: 25247424) »
Nascimento DS et al. Satellite Glial Cells Surrounding Primary Afferent Neurons Are Activated and Proliferate during Monoarthritis in Rats: Is There a Role for ATF3? PLoS One9:e108152 (2014).
Read more (PubMed: 25247596) »